Journal: Nuclear Receptor
Article Title: PLZF is a negative regulator of retinoic acid receptor transcriptional activity
doi: 10.1186/1478-1336-1-6
Figure Lengend Snippet: Structure and properties of the bait RAR mutant and of one of the identified preys, PLZF. A) Schematic representation of the nuclear receptor RARα and structural localization of the two mutations K262A and K244A. These mutations weaken the interaction with the corepressor SMRT and abolish the interaction with the coactivator SRC-1, as visualized by GST pull-down assays (insert). B) Structure of the transcription factor PLZF identified by the two-hybrid screening of an ovary cDNA library with pLex12-RAR K244A-K262A used as a bait.
Article Snippet: An ovary cDNA library (in pACT2 vector, Clontech) was screened using the L40a yeast strain transformed with the pLex10-RARK244A-K262A vector, essentially as described in [ ].
Techniques: Mutagenesis, Two Hybrid Screening, cDNA Library Assay